Protective effect of prolactin and placental lactogen on NO-induced Nb2 lymphoma cell apoptosis

FERNÁNDEZ ML, IGLESIAS MM, BIRON VA, WOLFENSTEIN-TODEL C

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Elsevier

Año: 2003 vol. 416 p. 249 - 256

0003-9861

Nitric oxide (NO) is an important modulator involved in immune regulation. Here,we describe conditions under which NOdonorsinduce apoptosis on Nb2 lymphoma cells,as evidenced by decreased cell viability and increased hypodiploid DNA contentdetermined by flow cytometry. In addition,DNA fragmentation typical of apoptosis was shown by agarose gel electrophoresis. Thisapoptosis was accompanied by a significant increase of caspase-3-like enzymatic activity. Both ovine prolactin (oPRL) and ovineplacental lactogen (oPL) exerted a protective effect on the NO-donor-induced apoptosis. Furthermore,dexamethasone (Dex)-induced cell death was also associated with caspase-3-like activity and oPL had the same potency as oPRL in its protective effect onDex-induced apoptosis of Nb2 cells.