Automated image analysis for monitoring oxidative burst in macrophages

MOLINARI, B.; TASAT, D.; FERNÁNDEZ, M.L.; DURÁN, H.; CURIALE J.; STOLIAR, A.; CABRINI, R.L

ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY / THE INTERNATIONAL ACADEMY OF CYTOLOGY [AND AMERICAN SOCIETY OF CYTOLOGY.

Analytical and Quantitative Cytology and Histolology

Año: 2000 vol. 22 p. 423 - 427

0884-6812

OBJECTIVE: To evaluate oxidative bursts induced by phorbol myristate acetate in phagocytes at the single-cell level by automated image analysis. STUDY DESIGN: The generation of reactive oxygen species was quantitatively expressed by means of histograms displaying the percentage of cells corresponding to each of the total optical densities measured. RESULTS: Macrophage subpopulations were quantitatively defined. This method allows detailed analysis of the amount of formazan per cell and the sites of deposition of blue precipitates in the cell. CONCLUSION: Image analysis is a reliable quantitative, single-cell assay for studying various cellular characteristics associated with macrophage functions