Mce2R from Mycobacterium tuberculosis represses the expression

SANTANGELO, MARÍA DE LA PAZ; BLANCO, FEDERICO; CAMPOS, ELEONORA; SORIA, MARCELO; BIANCO, VERÓNICA; KLEPP, LAURA; ALITO, ALICIA; ZABAL, OSVALDO; CATALDI, ANGEL; BIGI, FABIANA

Tuberculosis (Edinb)

Elsevier

Lugar: Amsterdam; Año: 2009 vol. 89 p. 22 - 28

1472-9792

The mce2 operon is one of the four mce operons present in Mycobacterium tuberculosis that encode exported proteins with a probable role in the virulence mechanisms of this bacterium. In the present study we demonstrated that Rv0586, which encodes a putative GntR-like regulator, is part of the mce2mce2 operon is one of the four mce operons present in Mycobacterium tuberculosis that encode exported proteins with a probable role in the virulence mechanisms of this bacterium. In the present study we demonstrated that Rv0586, which encodes a putative GntR-like regulator, is part of the mce2Rv0586, which encodes a putative GntR-like regulator, is part of the mce2 operon. By using a promoter–lacZ fusion approach and bioinformatics tools, we found that Rv0586lacZ fusion approach and bioinformatics tools, we found that Rv0586 represses the expression of Mce2 proteins and of a putative endonuclease IV, encoded by end (Rv0670) gene. For this reason, we have re-named the repressor protein Mce2R. By gel-shift experiments Mce2R binding was determined to be located within the mce2 promoter region. In addition, two FadR-like operator motifs were identified within the promoter regions of both the mce2 operon and the end gene. These motifs overlap putative 10 and 35 promoter boxes. M. tuberculosis carrying mce2 and endend (Rv0670) gene. For this reason, we have re-named the repressor protein Mce2R. By gel-shift experiments Mce2R binding was determined to be located within the mce2 promoter region. In addition, two FadR-like operator motifs were identified within the promoter regions of both the mce2 operon and the end gene. These motifs overlap putative 10 and 35 promoter boxes. M. tuberculosis carrying mce2 and endmce2 promoter region. In addition, two FadR-like operator motifs were identified within the promoter regions of both the mce2 operon and the end gene. These motifs overlap putative 10 and 35 promoter boxes. M. tuberculosis carrying mce2 and endmce2 operon and the end gene. These motifs overlap putative 10 and 35 promoter boxes. M. tuberculosis carrying mce2 and endM. tuberculosis carrying mce2 and end promoter–lacZ fusions were used to infect J774 macrophage-like cells. Expression of b-galactosidase was induced after phagocytocis, suggesting that some cellular factor could be a key component of the molecular switch regulation expression of the mce2 operon. In conclusion, these results add novel evidence of the complex regulation of mce operon expression.lacZ fusions were used to infect J774 macrophage-like cells. Expression of b-galactosidase was induced after phagocytocis, suggesting that some cellular factor could be a key component of the molecular switch regulation expression of the mce2 operon. In conclusion, these results add novel evidence of the complex regulation of mce operon expression.mce2 operon. In conclusion, these results add novel evidence of the complex regulation of mce operon expression.mce operon expression.