STUDY OF BIOLOGICAL ACTIVITIES IN THE FIRST TRIPSIN INHIBITOR ISOLATED FROM CHAÑAR SEEDS

BRENDA OZÓN; CANDELA SAMAN; EVELINA QUIROGA; FLORENCIA GEIER; SANTIAGO CLAVER; WALTER DAVID OBREGÓN; MARTINA ROSSOTTI; ANA LAURA VICARIO; JULIANA COTABARREN

TAMAULIPAS

Congreso; 8vo SOMECTA Congreso Internacional de Ciencia y Tecnología Agropecuaria; 2021

Universidad Autónoma de Tamaulipas

Protease peptide inhibitors (PPIs) are low molecular weight chemical compounds found in tissues and fluids of animals, plants and microorganisms. The potential and therapeutic efficacy of PPIs have been reported for different diseases. Despite this, its use as inhibitors of enzymes involved in metabolic disorders has been little studied, so the search for new alternative agents of natural origin that can be applied in such pathologies becomes relevant. This study presents the isolation and purification of the first Geoffroea decorticans thermostable trypsin inhibitor (GdTI), a protein of 6743.7 Da with strong inhibitory activity (Ki =2.1 nM) even after being subjected to high extreme temperatures and pHs (100% activity after 5 h to 100°C and 80% activity after 1 h to pH 2-12), representing one of the most potent serine protease inhibitors of plant origin. In addition, it was possible to show that GdTI has anticoagulant activity for the intrinsic and extrinsic coagulation pathways, being the first inhibitor of plant origin with effect on the extrinsic coagulation pathway. Finally, GdTI showed inhibitory activity of α-glucosidase (CI50 =0.18 μM) showing its potential as a hypoglycemic agent. The results of this work suggest that GdTI may be used as a natural antidiabetic drug, due to its antithrombotic and hypoglycemic activity, encouraging future studies with an impact on its potential biomedical application