Automation of a Solid Phase Extraction Method for the Determination of Ochratoxin A in Wine and Beer Samples Prior to LC-MS/MS

C. CROES, CERUTTI, SOLEDAD, ENZWEILER, TOM, GAIRLOCH, ELENA, KERO, FRANK, MARIÑO REPIZO, LEONARDO, SENIOR, ADAM, VANDELL, VICTOR

Chicago, Illinois

Conferencia; PITTCON; 2014

PITTCON

The emerging threats of mycotoxin contamination in our food, particularly ochratoxin A (OTA), have led to surveillance programs employing low-level screening methods in both industrial and government foods safety laboratories. The persistent nature of this contaminant has led to its determination in a range of processed foods including cereals, beer and wine. Of particular interest to this investigation is the utility of automation to facilitate large scale quality control efforts in the preparation of samples prior to analysis by liquid chromatography-mass spectrometry-mass spectrometry (LC-MS/MS). A solid phase extraction method using application specific polymer column technology called the ISOLUTE Myco (60 mg/3 mL column) was developed from a multivariate statistical design of experiment (DOE). This method was employed to determine OTA concentrations in Malbec wines from Argentina. The relative recovery for the extracted mycotoxin was 95-105% (