INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
On-line solid phase extraction of Cd from protein fractions of serum using oxidized carbon nanotubes coupled to electrothermal atomization atomic absorption spectrometry.
Autor/es:
ACOSTA M; SAVIO M; TALIO MC; FERRAMOLA ML; GILA RA; MARTINEZ LD
Revista:
MICROCHEMICAL JOURNAL
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 p. 94 - 98
ISSN:
0026-265X
Resumen:
This work presents a sensitive and accurate method for Cd determination in serum protein fractions separated by
cellulose acetate electrophoresis (CAE). The determinations were carried out by on-line solid phase extraction
using oxidized carbon nanotubes coupled to electrothermal atomization atomic absorption spectrometry
(ETAAS). The detection and quantification limits were 10 ng L−1
and 35 ng L−1
respectively with a precision of
4.0% as relative standard deviation. This procedure joined the simplicity of protein separation by CAE, the
preconcentration properties of solid phase extraction using ox-CNT and the excellent sensitivity attainable by
ETAAS. The method was applied to samples with biological relevance (humans and experimental rats). Our
work demonstrated that Cd could be determined in serum protein fractions separated by electrophoresis with
good accuracy using the proposed method.