Modified magnetic nanoparticles in an electrochemical method for the ochratoxin A determination in Vitis vinifera red grapes tissues

MARTÍN A. FERNÁNDEZ BALDO; FRANCO A. BERTOLINO; GERMÁN A. MESSINA; MARÍA I. SANZ; JULIO RABA

TALANTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2010 vol. 83 p. 651 - 657

0039-9140

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} span.shorttext1 {mso-style-name:short_text1; mso-ansi-font-size:14.5pt; mso-bidi-font-size:14.5pt;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> This work described the developed and characterized an electrochemical method combined with the use of modified magnetic nanoparticles (MNPs), which show a rapid and sensitive determination of ochratoxin A (OTA) in wine grapes (Cavernet Sauvignon, Malbec and Syrah) post-harvest tissues. The wine grapes were inoculated with Aspergillus ochraceus to obtain OTA in artificially infected samples. The OTA was directly determined using square-wave voltammetry. The current obtained is directly proportional to the concentration of OTA present in the samples. This method has been used for OTA determination in wine grapes and it was validated against a commercial ELISA test kit. The limits of detection calculated for electrochemical detection and the ELISA were 0.02 and 1.9 μg kg-1, respectively and the coefficients of variation for accuracy and precision dates were below 5.5%. This method promises to be suitable for the detection and quantification of OTA in apparently healthy fruits post-harvest for assuring safety and quality of food as well as consumer’s health.