INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
Modified magnetic nanoparticles in an electrochemical method for the ochratoxin A determination in Vitis vinifera red grapes tissues
Autor/es:
MARTÍN A. FERNÁNDEZ BALDO; FRANCO A. BERTOLINO; GERMÁN A. MESSINA; MARÍA I. SANZ; JULIO RABA
Revista:
TALANTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2010 vol. 83 p. 651 - 657
ISSN:
0039-9140
Resumen:
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This work described the developed and characterized an electrochemical method combined with the use of modified magnetic nanoparticles (MNPs), which show a rapid and sensitive determination of ochratoxin A (OTA) in wine grapes (Cavernet Sauvignon, Malbec and Syrah) post-harvest tissues. The wine grapes were inoculated with Aspergillus ochraceus to obtain OTA in artificially infected samples. The OTA was directly determined using square-wave voltammetry. The current obtained is directly proportional to the concentration of OTA present in the samples. This method has been used for OTA determination in wine grapes and it was validated against a commercial ELISA test kit. The limits of detection calculated for electrochemical detection and the ELISA were 0.02 and 1.9 μg kg-1, respectively and the coefficients of variation for accuracy and precision dates were below 5.5%. This method promises to be suitable for the detection and quantification of OTA in apparently healthy fruits post-harvest for assuring safety and quality of food as well as consumers health.