INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
Determination of the β-Glucosidase Activity in Different Soils by Pre Capillary Enzyme Assay Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection
Autor/es:
PATRICIA W. STEGE; GERMÁN A. MESSINA; GUILLERMO BIANCHI; ROBERTO A. OLSINA
Revista:
JOURNAL OF FLUORESCENCE
Editorial:
SPRINGER/PLENUM PUBLISHERS
Referencias:
Año: 2010 vol. 20 p. 517 - 523
ISSN:
1053-0509
Resumen:
Abstract Enzyme activities can provide indication forquantitative changes in soil organic matter (SOM). It isknown that the activities of most enzymes increase as nativeSOM content reflecting larger microbial communities andstabilization of enzymes on humic materials. β-Gucosidase(β-Glu) activities have been frequently used as indicators ofchanges in quantity and quality of SOM. In this study wepropose a simple and very sensitive method, which has lowerlimit of detection compared with classic spectrophotometricmethod with the aim of determinate the β-Glu activity in soilsamples using Fluorescein mono-β-D-glucopyranoside(FMGlc) as a substrate. The fluorescein released by theenzymatic reaction was quantified by capillary electrophoresislaserinduced fluorescence (CE-LIF) method. The backgroundelectrolyte (BGE) consisted in 40mMphosphate buffer, pH 6.The LOD and LOQ for fluorescein were 1.3 10−7 mg mL−1and 6.4 10−6 mg mL−1, respectively. This work deals withthe minimization of the mixture for the enzymatic reactionand with the optimization conditions of CE separation. Tothe best of our knowledge, this is the first time that anenzymatic activity was detected in soil using CE-LIF system.