Lipase production by Penicillium restrictum using solid waste of the industrial babassu oil production.

D.M.GUIMARAES FREIRE, A.K. GOMBERT, A.LOPES, KARINA HERRERA SEITZ, MARCIA B. PALMA, S. KIVATINITZ, L.R. CASTILHO

University Park Holiday Inn, Fort Collins, Colorado

Simposio; Symposium on Biotechnology for fuels and chemicals; 1999

Large amounts of industrial proceses use SSF technology to obtain interesting products for the humanity such as foods, organic acids, encimes and compostage of industrial and domestic waste. The main objective of the present work is to compare the production, in SSF, of lipases and another hydrolases by a strain of Penicillium restrictum by using different conditions of medium supplementation. A solid waste of industrial production of babassu oil, from which the strain used has been isolated, was employed as the nutrient source. This medium was inoculated with 1x106 spores per g of substrate and supplemented with peptone, olive oil or Tween 80 (1% w/v). The moisture of the different medium was 70%. Experiments were conducted for 63 h/30° C under static conditions with forced aeration using humidified air. In all the medium were determined moisture level, pH, lipidic material consume, biomass (expressed like glucosamine) and enzymatic activity (lipase, protease and glucoamylase) produced during the cultivation (14, 24, 38, 48, and 63 hours). In all cases, supplementation enhanced fungal growth and enzyme synthesis. A peak of lipase activity was observed after 24 hours of cultivation, except for the medium supplemented with Tween, in which lipolytic production occurred earlier (20 hours). The medium supplemented with peptone provided the maximum lipase activity (28 U/g). The amylase activity was highest (31,8 U/g) in the medium containing olive oil and the proteases were most intensively produced (8,6 U/g) with Tween supplementation. Comparing the enzyme production by this strain of P.restrictum in submerged and solid-state fermentations, it can be concluded that the latter presents a greater technological potential for obtaining extracellular enzymes, such as lipases, proteases and amylases.