High passage numbers induce resistance to apoptosis in C2C12 muscle cells

LUCIA PRONSATO; ANABELA LA COLLA; ANA RONDA; LORENA MILANESI; RICARDO BOLAND; ANDREA VASCONSUELO

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 p. 1 - 9

0327-9545

ABSTRACT:Cell lines with high passage numbers exhibit alterations in cell morphology and functions. Inthe present work, C2C12 skeletal muscle cells with either low (60) passage numbers (identifiedas l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2as a functionof culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA(mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential,we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We proposeserially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtainedin this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.*(corresponding author: Andrea Vasconsuelo)