Relation between respiratory activity and sperm parameters in boar spermatozoa cryopreserved with alpha-tocopherol add selected by Sephadex

M. M. SATORRE; E. BREININGER; P. CETICA; M. CÓRDOBA

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2018 vol. 53 p. 979 - 985

0936-6768

Our aim was to evaluate the effect of Sephadex filtration on respiratory activity of porcine spermatozoa and its relation with quality and functional sperm parameters. Samples were evaluated regarding oxygen uptake and sperm parameters: motility, plasma and acrosome membrane integrity, capacitation and acrosome reaction induction in vitro, plasma membrane functionality,determined by the hypo-osmotic swelling test (HOST), and lipid peroxidation assessed by thiobarbituric acid assay. Sephadexfiltration improved allroutine quality parameters (motility, plasma and acrosome membrane integrity) and functional parameters (HOST, in vitro capacitation and true acrosome reactionlevels) and produced a significant decrease in cryocapacitation and lipid peroxidation. Oxygen uptake increased in Sephadex samples(41 ± 7%) respect to single washing. Oxygen addition of carbonyl-cyanide-m-chlorophenylhydrazone (CCCP) confirmed mitochondrial coupling in washed and Sephadex samples;showingan increase of 2.6 and 4.2 times for oxygen consumption in single washing and Sephadex ones, respectively. The increase in oxygen uptake with succinate addition with respect to basal oxygen uptake was significantly lower in Sephadex samples (63 ± 25%) than in the washed ones (183 ± 35%). Sephadex samples showed higher mitochondrial activity measured by oxygen consumption and improved quality and functional parameters. Our study recommends this protocol due to the fact that this filtration method removes dead or damaged spermatozoa allowing to obtain cryopreserved boar spermatozoa with optimized fertilizing capacity.