INVESTIGADORES
CETICA Pablo Daniel
artículos
Título:
Reactive Oxygen Species Production and Redox state in Parthenogenetic and Sperm mediated bovine oocyte activation
Autor/es:
S. MORADO; P. CETICA; M. BECONI; J. THOMPSON; G. DALVIT
Revista:
REPRODUCTION
Editorial:
BIOSCIENTIFICA LTD
Referencias:
Lugar: Bristol; Año: 2013 vol. 145 p. 471 - 478
ISSN:
1470-1626
Resumen:
The knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen?thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7-24 h from activation) by 2´,7´-dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P
