Pentose phosphate pathway activity: effect on in vitro maturation and oxidative status of bovine oocyte

C. GUTNISKY; G. DALVIT; J. THOMPSON; P. CETICA

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2014 vol. 26 p. 931 - 942

1031-3613

The relationship between the pentose phosphate pathway (PPP) activity in COCs and oxidative and mitochondrial activity in bovine oocytes was evaluated, with the aim of analyzing the impact of two inhibitors (NADPH, 6-AN) and a stimulator (NADP) of the key enzymes of PPP on the maturation rate, the oxidative and mitochondrial activity, and the mitochondrial distribution in oocytes. The proportion of COCs with measurable PPP activity, (assessed using BCB staining), glucose uptake, lactate production, and meiotic maturation rate diminished when 6-AN was added to the maturation media (p