Effect of lactate dehydrogenase activity and isoenzyme localization in bovine oocytes and utilization of oxidative substrates on in vitro maturation

P. CETICA, L. PINTOS, G. DALVIT AND M. BECONI

THERIOGENOLOGY

Elsevier

Año: 1999 vol. 51 p. 541 - 550

0093-691X

 Oocyte nutritional metabolism changes during maturation in order to increase the energy available to support metabolic requirements. The aim of this work was to study pyruvate and lactate utilization as oxidative substrates on IVM and lactate dehydrogenase (LDH) activity and localization of their isoenzymes in bovine oocytes. Immature cumulus-oocyte complexes (COCs) were recovered by aspiration of antral follicles in ovaries obtained from slaughtered cows. The COCs and denuded oocytes were separately cultured in TCM-199 with steer serum (controls) and were supplemented with pyruvate, lactate or lactate plus NAD for 24 h at 39°C in 5% COf95% humidified air. No significant differences were found in IVM rates of COCs matured according to the various treatments (P>0.05). The 1VM rate in denuded oocytes without supplementation was 47.8%. The presence of pyruvate in the culture medium resulted in an increased number of matured denuded oocytes (59.4%; P< 0.05), but the addition of lactate failed to improve the IVM rate of matured denuded oocytes (47.6%, P>0.05). When the medium was supplemented with lactate plus NAD, the IVM rate of denuded oocytes Iikewise failed to differ from that obtained with the addition of pyruvate (59.9%, P>0.05). The LDH activity in immature and matured COCs and denuded oocytes was (3.1 ± 1.6) 10-3, (3.3 ± 1.6) 10-3 UlCOC, (5.2 ± 2.0)10-5, (5:4 ± 3.5) 10-5U/oocyte with pyruvate as substrate, and (1.2 ± 0.5) 10-3, (1.0 ± 0.5) 10-3 UlCOC, (2.2 ± 0.1) 10-5, (2.5 ± 1.4) 10-5 Uloocyte respectively, with lactate; no significant differences due to maturation status were observed (P>0.05; n = 9 for each LDH activity). E1ectrophoresis disc10sed that the principal band corresponded to the LDH-1 isoenzyme in oocytes, while there was no predominance of any isoenzyme in cumulus cells. Due to the fact that LDH-1 is the main oocyte isoenzyme, the pyruvate used during oocyte maturation could be partly produced from lactate when the NAD supply is adequate. Cumulus cells would be responsible for providing pyruvate and/or lactate as oxidative substrates to be used by the bovine oocyte and this supply would be regulated by the LDH activity in these cells.