Treatment with triyodotironine (T3) produces changes in the expression of pro-angiogenic factors in endometrial human Ecc-1 cell line.

EZQUER M; HAPON MB; RINALDINI E; CAMPO VERDE ARBOCCO F; EZQUER M; GAMARRA LUQUES C

Congreso; IV Reunión Conjunta de las Sociedades de Biología de la República Argentina.; 2020

Embryonic implantation takes place in a hypoxic environment, where HIF-1Α, the main regulator of the cellular response to hypoxia, together with estradiol (E2) and progesterone (P4), promote transcription of genes involved in angiogenesis and vascularization. VEGFA is the main modulator of these processes, and its action in the endometrium complements and coordinates with other pro-angiogenic factors. Thyroid hormones (TH) are essential during implantation and the early stages of embryonic development. Our laboratory demonstrated that, in addition to the alterations in hormonal metabolism and ovarian function, hypothyroid rats show decreased uterine vascular density during implantation, and that T3 treatment prior to implantation significantly increases Vegfa mRNA expression in this group. Uterine vascularization plays a critical role in the success of pregnancy, so the aim of this work was to investigate, the mechanism by which THs participate in the process of angiogenesis and endometrial vascularization using an in vitro model. For this purpose, preconditioned ECC-1 cell line (human endometrial) with CoCl2 to simulate the condition of hypoxia, and further stimulated with E2+P4 or E2+P4+hCG (simulating the pre-implantation and implantation phases respectively), were treated with or without T3. The expression of HIF-1Α and VEGFA, in the cell lysate and the secretome, respectively, were determined by ELISA. The relative expression of mRNA of angiogenic factors: HIF-1A, VEGFA, bFGF, FGFR, EDN1, ANGPT1 and PDGF, was analyzed by RT-qPCR. The ELISA test showed that the preconditioned cells showed a significant increase in the expression of HIF-1Α compared to the control (p