Caracterización de las células MEPC 5 (Mouse Epididymal Proximal Caput 5) como modelo para el estudio de la expresión epididimaria de pedf (factor derivado del epitelio de pigmentario)

LOPEZ ME; MONCLUS M,; PIETROBON E; CABRILLANA ME

mendoza

Jornada; jornadas de Investigación 2019 en la Facultad de Ciencias Médicas.; 2019

UNIVERSIDAD NACIONAL DE CUYO

PEDF (pigment epithelium derived factor), is a glycoprotein that belongs to the serine proteases family, widely spread in different mammalian tissues. It has functions in cell survival and repair, as an antioxidant, antiapoptotic and antiangiogenic factor antagonizing VEGF (Vascular Endothelial Growth Factor). There many antecedents regardin PEDF rol in female reproductive tract as an antioxidant and antiapoptotic factor protecting the ovarian granullosa cells. Recently has begun its study in physiological and pathological situations in male reproductive system. Our laboratory recently described PEDF expression and its androgen-dependent in the male tract of Wistar rats. In this specie PEDF is expressed mainly in epididymal, seminal vesicles and prostate but notably not in testes. We extended the study to an in vivo model (C57bl/6 wild type mice) and also in a cell culture model MEPC5 cells obtained and immortalized from C57bl/6 mice. MEPC5 cells shown PEDF, P21 and Estrogen receptor alpha (ER α) expression by immuno cytochemistry. Androgen receptor (AR), VEGF (a known PEDF antagonist) were also detected by western blot and indirect immunofluorescence (IFI). In mouse tissues we observed PEDF expression over testicular peritubular cells as was described previously en human and primates but no in rats. These results favour the development of new lines of research to recognize the functional role of PEDF in the reproductive tract of rodents.