Consumption of organosulphur compounds derived from garlic macerated oil as strategy to prevent the development of cardiovascular diseases

CAROLINA TORRES; LUCIANA MAZZEI; CLAUDIA CASTRO; MATILDE DE PAOLA; MIGUEL FORNÉS; MARCOS ARLANDI; WALTER MANUCHA; DARÍO CUELLO-CARRIÓN; ALEJANDRA CAMARGO

Parma

Congreso; 3rd International Conference on Food Bioactives & Health; 2020

International Society of Food Bioactives & Health

Numerous authors argued that garlic phytochemicals have the potential to prevent or improve Cardiovascular Diseases (CVD). That evidence was allowed employing allicin or S-allyl cysteine preparations. Previously we found that 2-vinyldithiin (2VD), an organosulfur compound derived from allicin, has a promising systemic bioavailability. These facts make it an interesting candidate to evaluate its bioactivity using in vivo models. Consequently, garlic oil macerate (GOM), which possesses high concentrations of 2VD, was chosen. The objective was to investigate the impact of GOM on the development of CVD in an experimental animal model. Parameters linked with atherosclerosis and transcription factor sensitive to oxidative/inflammatory disorders as Wilms tumor (WT1), were evaluated. GOM (50 g of raw chopped garlic/ 500 mL of sunflower oil (SO) containing 350 ppm of 2VD, 24 ppm of ajoene, 34 ppm of diallyl sulfide and 28 of diallyl trisulfide, was used for the experiments. Three groups of ApoE-KO (N=8), were randomize selected: Control (C): standard diet; Group (SO): diet+sunflower oil (3.3 mL/kg/day); and Group (GMO): diet+GMO. After two months of treatment, mice were sacrificed, and serum biochemical parameters were evaluated Markers of atherogenesis, inflammation and oxidative stress were studied in aortas and kidney. In renal tissue, the expressions of WT1, Hsp70 and the inducible isoform of nitric oxide synthase (iNOS) were also assessed. Our results showed a significant decrease in triglyceridemia in animals of GMO group, compared with SO group (36.9 mg% ± 6.3 vs 60.8 mg% ± 5.9; P