NOX5 expression levels in human monocytes and polymorphonuclear leukocytes in Non-alcoholic fatty liver disease: Tuning up the technique

ROXANA SILVA; ISABEL M. QUESADA

mendoza

Jornada; II Jornadas de Medicina Traslacional; 2019

Introduction and objectives: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disorder which is associated with accumulation of fats in the liver. NAFLD is strongly associated with features of the metabolic syndrome, including obesity, insulin resistance, type 2 diabetes (DM2) and dyslipidemia. This pathology, like other metabolic diseases, is associated with oxidative stress that leads to an excessive production of reactive oxygen species (ROS). The NADPH oxidases (NOX) take on a leading role in ROS production. NOXs are highly expressed in the plasma and lysosomal membranes of phagocytes, such as neutrophils and macrophages. While there is convincing evidence for a role of NOX1, NOX2 and NOX4, the role of NOX5 in disease is less clear. Here, we aimed at elucidating whether NOX5 is expressed in human monocytes (Mon) and polymorphonuclear leukocytes (PMN), and examine its potential correlation with the degree of steatosis in NAFLD.Methods: Mon and PMN from blood of control patients were isolated and purified using the Ficoll-Hypaque technique. RT-PCR, was utilized to examine NOX5 expression.Results: we could isolate mRNA from human Mon and PMN of control patients. We found that NOX5 is well expressed in both blood immune cells.Conclusions: once the technique is ready, we will begin the collection of samples from patients with NAFLD, hoping that NOX5 levels will be indicators of the stage of the disease.