CONICET | Buscador de Institutos y Recursos Humanos

Tumor stroma is one of the emerging topics of interestin cancer research. In the present work, we evaluated: 1) adiponectin, ADAMTS1and perilipin expression in conditioned media (CMs) of human adipose tissueexplants from normal (hRAN) and tumor (hRAT) kidney; 2) AdipoR1, ObR, adiponectinand perilipin expression in hRAN- and hRAT. We also evaluated changes in theexpression of: 1) AdipoR1, ObR, CD44 and vimentin in tumor (786-O, ACHN,Caki-1) and non tumor (HK-2) renal epithelial cells; 2) pERK/ERK and pPI3K/PI3Kchanges in cell lines incubated by 2 and 24h with hRAN-, hRAT, or control-CMs.Human renal adipose tissues were obtained frompatients with renal cell carcinoma (hRAT, n=10) and kidney donors (hRAN, n=11).The CMs of hRAN and hRAT were collected 24h post incubation. Protein expressionwas evaluated by IHQ and WB. The celllines were treated with CMs by 24h. Statistical differences were evaluated byone-way ANOVA or t-test, with Tukey´spost hoc tests. We observed a lower perilipinexpression in hRAT-CMs vs. hRAN-CMs; andwe found that adiponectin, AdipoR1, and perilipin expression were significantlyincreased in hRAN vs. hRAT explants(p<0.01). Also, we observed a decrease in the expression of AdipoR1 in ACHNand Caki-1 and the increased expression of ObR and CD44, when these cells wereincubated with hRAT-CMs compared to hRAN- and control-CMs (p <0.001). Inaddition, we observed an increase in the expression of vimentin in HK-2, whenthese cells were incubated with hRAT-CMs. Further, we found an increase in theexpression of pERK and pPI3K in HK-2 and 786-O, when these cells were incubatedwith hRAT-CMs compared to hRAN- and control-CMs (p <0.001). In conclusion,changes observed in the adipose microenvironment could be favoring tumorprogression and therefore, the tumor stroma should be taken into considerationwhen dealing with a malignancy.