PARTICIPATION OF HUMAN ADIPOSE TISSUE IN THE REGULATION OF TUMOR PROGRESSION IN KIDNEY CANCER

ROMEO, LEONARDO R; LÓPEZ-LAUR, JD; BRUNA, FLAVIA; SASSO, CORINA V.; CONTADOR, DAVID; CARÓN, RUBÉN W.; SANTIANO, FLAVIA E.; LÓPEZ FONTANA CM; CAMPO VERDE ARBOCCO F; GÓMEZ, SILVINA E; ZYLA, LEILA E; PISTONE CREYDT, VIRGINIA

Merlo, San Luis

Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017

We have recently demonstrated that human adipose tissue of tumor kidney [hRAT],showed leptin and versican overexpressed compare to adipose tissue of normalkidney [hRAN]. Meanwhile, adiponectin and perilipin were significantlyincreased in hRAN. Conditioned media (CMs) of hRAN significantly increasedproliferation of tumor [ACHN] and non-tumor [NK-2] renal epithelial cells. Now,we evaluated the expression of receptors (R): adiponectin -1, -2 (AdipoR1/R2)and leptin (ObR) in hRAN vs. hRAT andACHN vs. HK-2. Additionally, CD44expression (homing cell adhesion molecule) was assessedin cells. Human renal adipose tissues were obtained from patients with renalcell carcinoma [hRAT, n=6] and kidney donors [hRAN, n=8]. The CMs of hRAN andhRAT were collected 24h post incubation and cells were treated with CMs by 24 h.Protein expression was evaluated in tissue by IHQ, and WB in cells. Statisticaldifferences among experimental conditions were evaluated by one-way ANOVA or t test, with Tukey´s post hoc tests. We found AdipoR1increased and ObR decreased in hRAN vs.hRAT (p<0.05). In addition, ACHN and NK-2 incubated with hRAT- showed CD44 and ObR increased vs.hRAN- and control-CMs (p<0.05). Further, AdipoR1 was significantly lower inACHN incubated with hRAT-CMs compared to hRAN-CMs (p<0.05). And AdipoR1 decreasedin hRAT- and hRAN-CMs vs. control-CMs(p<0.001). No significant changes were observed in AdipoR2 between the celllines with different CMs. In conclusion, changes observed in the adiposemicroenvironment could be favoring tumor progression and therefore, the tumorstroma should be taken into consideration when dealing with a malignancy.