Chemical characterization and biological activities of Baccharis grisebachii lyophilized decoction.

GOMEZ J; PAREDES JD; LUNA L; FERESIN GE; SIMIRGIOTIS MJ; GAMARRA LUQUES C; WENDEL GH; TAPIA A; VILLEGAS GABUTTI CM; BORQUEZ J; MARIA AO

Mendoza

Congreso; XXXVI Reunión Anual de la Sociedad de Biología de Cuyo.; 2018

Sociedad de Biologia de Cuyo

The decoction from Baccharis grisebachii is used as a digestive, to relieve gastric ulcers, cicatrizing and antiseptic in Argentine. A lyophilized decoction (LD) from the aerial parts was evaluated regarding their anti-ulcer, antioxidant and cytotoxic activities and UHPLC-MS metabolome fingerprinting. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The LD showed a moderated free radical scavenging activity in the DPPH (IC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). At a dose of 750 mg/kg, the LD had the highest gastroprotective effect with a ninety-three percent inhibition of damage through mechanism that involve NO and prostaglandins using the rat model of ethanol-induced gastric damage. On the other hand, LD does not induce cytotoxic changes on human tumor and no-tumor cell lines at concentrations assayed. Thirty one compounds were detected and identified based on the UHPLC OT-MS including, nine correspond to flavonoids, thirteen to caffeoyl-quinic acid derivatives, three to coumarins and three to other different phenolic compounds. The results support that Baccharis grisebachii decoction is a valuable natural product with potential to improve human health.