Nano-formulated anandamide decreases neuro-inflammatory markers in spontaneously hypertensive rats.

DIEGO KASSUHA; EMILIANO DIEZ; WALTER MANUCHA; GARCÍA SEBASTIÁN; VIRNA MARTÍN GIMENÉZ; ROBERTO YUNES; MANUEL GUEVARA; FERES MOCAYAR

Córdoba

Congreso; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias.; 2018

Sociedad Argentina de Investigación en Neurociencias (SAN)

Essential hypertension is responsible for almost 95% of all cases of hypertension. Frequently of neurogenic origin, it is linked with an over-excitation of brainstem centres, sympathetic hyperactivation and imbalance in the levels of pro- and anti-inflammatory cytokines. Spontaneously hypertensive rats (SHR) is a validated model of hypertension, plus several neurocognitive deficits. Since endocannabinoid anandamide (AEA) protects neurons from the inflammatory damage, and cannabinoid signalling decreases in brains of hypertensive animals, we applied a nano-formulated AEA in SHR. We used adult male rats (n=7) of 250-300 g normotensive (WKY) and hypertensive (SHR), treated or not with nano-formulated AEA in polycaprolactone (AEA/PCL), at a weekly dose of 5 mg/Kg IP, for four weeks. Regarding WKY, the SHR showed elevated inflammatory markers (IL-1, IL-6, FNTα, ultrasensitive PCR and plasma Hsp70, p