Nephrogenic factors pattern during the hypertension development

DOCHERTY NEIL G; MANUCHA W; MAZZEI LUCIANA

MERLO, SAN LUIS

Jornada; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017

Sociedad de Biología de Cuyo

Spontaneously hypertensive rats (SHRs) develop hypertension at about 6 weeks of age without physiological, pharmacological or surgical intervention. Hypertension development amplifies the risk of onset and progression of kidney disease. In addition, the altered nephrogenic process has been recognized as a powerful risk factor for kidney disease, leading to hypertension; this makes it controversial to analyze whether hypertension is a cause or a consequence of kidney disease. Therefore, the goal of this study was to evaluate the expression of nephrogenic genes, like Wilms? tumor transcription factor 1 (WT-1) and related genes, such as heat shock protein 70 (Hsp70) and vitamin D receptors in connection with the development of hypertension. Methods: Male SHR and control rats (N=40 per group) were evaluated from birth until week 8 of life. Weekly, we took blood pressure and 5 rats per group were sacrificed. The kidneys were removed and weighed. Sections of the renal cortex were processed for histological and molecular studies. Results: The elevation of systolic blood pressure was significant since week 6. Histology showed a slight increase in deposits of collagen fibers since week 4. Additionally, in kidney cortices, the expression of WT-1, Hsp70 and vitamin D receptors decreased since week 4 (previous to the increase in blood pressure). The current results would suggest that hypertension may be a result of possible changes in renal development, evidencing the importance of nephrogenic factors in the hypertension development.