Fibrosis and apoptosis gene pattern in neonatal obstructive nephropathy: Rosuvastatin modulation.

LUCIANA MAZZEI; ISABEL MERCEDES GARCÍA; MARÍA EUGENIA BENARDÓN; VALERIA CACCIAMANI; PATRICIA G. VALLES; WALTER MANUCHA

Universidad de la Ciudad de La Punta San Luis

Congreso; XXVII Reunión Científica Anual Sociedad de Biología de Cuyo; 2009

Sociedad de Biología de Cuyo

Obstructive nephropathy is a kidney disorder complex to treat due to severe apoptosis and fibrosis. Previous studies showed that rosuvastatin (Ro) may have potential utility as a therapeutic option in kidney diseases which lead to apoptosis and fibrosis. The goal was to evaluate the possible antifibrotic and antiapoptotic effects of Ro during neonatal unilateral ureteral obstruction (UUO). Neonatal rats were surgically obstructed (experimental group) or not (control group), and Ro treated or not (10 mg/kg per day) during 14 days. Subsequent nephrectomy and processing of the renal cortex were performed to determine, using RT-PCR technique, gene expression of inducible nitric oxide synthase (iNOS), heat shock factor 1 (hsf1), heat shock protein 70  (hsp70), bax, bcl2, wt1, p53, snail, bone morphogenetic protein (bmp7), E-cadherin, transforming growth factor (tgf-â) and tumor necrosis factor (tnf-á). UUO induced fibrosis and apoptosis, while Ros treatment modulated the fibrotic and apoptotic gene pattern and increased the bmp7, caderina E, wt1, p53 and bcl2 expression as well as decreased the profibrotic and proapoptotic genes expression (bax, tnf-á y tgf-â). Our results allow us to suggest that Ro renal protection during UUO is linked to heat shock response and nitric oxide bioavailability interaction, with concomitant decrease in pro apoptotic gene pattern.