REPELLENT ACTIVITY OF ESSENTIAL OIL FROM Artemisia absinthium (ASTERACEAE) AGAINST ADULTS OF Tribolium castaneum (INSECTA, COLEOPTERA: TENEBRIONIDAE)

STEFANAZZI1, N., GUTIERREZ, M.M., STADLER, T. Y FERRERO, A.A.

Huerta Grande, Córdoba.

Otro; Primera Reunión Conjunta de Sociedades de Biología de la República Argentina.; 2008

Sociedades de Biología de la República Argentina

BIOCELL 32(1), 2008 ABSTRACTS 145 261. REPELLENT ACTIVITY OF ESSENTIAL OIL FROM Artemisia absinthium (ASTERACEAE) AGAINST ADULTS OF Tribolium castaneum (INSECTA, COLEOPTERA: TENEBRIONIDAE)(ASTERACEAE) AGAINST ADULTS OF Tribolium castaneum (INSECTA, COLEOPTERA: TENEBRIONIDAE)Tribolium castaneum (INSECTA, COLEOPTERA: TENEBRIONIDAE) Stefanazzi N1,3, Gutierrez MM1, Stadler T2, Ferrero AA1.1,3, Gutierrez MM1, Stadler T2, Ferrero AA1. 1Dto. de Biología, Bioquímica y Farmacia, UNS. San Juan 670, (8000) Bahía Blanca. 2 Laboratorio de Investigaciones y Servicios Ambientales (LISAMEN) Mendoza. 3Becario CONICET. E-mail: nstefanazzi@uns.edu.arDto. de Biología, Bioquímica y Farmacia, UNS. San Juan 670, (8000) Bahía Blanca. 2 Laboratorio de Investigaciones y Servicios Ambientales (LISAMEN) Mendoza. 3Becario CONICET. E-mail: nstefanazzi@uns.edu.ar2 Laboratorio de Investigaciones y Servicios Ambientales (LISAMEN) Mendoza. 3Becario CONICET. E-mail: nstefanazzi@uns.edu.ar3Becario CONICET. E-mail: nstefanazzi@uns.edu.ar The repellent activity of essential oil from Artemisia absinthiumArtemisia absinthium was evaluated against adults of Tribolium castaneum. The repellency test was conduced using an experimental arena consisting of in five circular glass boxes, the central box was united to the other boxes with glass tubes, diagonally. The samples and the controls were distributed respectively in two opposed boxes containing 4 g of treated and untreated diet with solvent alone. The solvent used was hexane. In the central box forty adults were released. After 24 h the number of adults present at the boxes were counted. Each concentration was tested three times. Preference Index was calculated as PI= (% of insects at treated diet)-(% of insects at untreated diet) / (% of insects at treated diet)+(% of insects at untreated diet) where PI = -1 to – 0,10, repellent plant; PI= -0,10 to +0.10, Neutral plant and PI= +0,10 to +1, attractant plant. At 0.04% PI was -0.42 and at 0.4 PI was -0.77.Tribolium castaneum. The repellency test was conduced using an experimental arena consisting of in five circular glass boxes, the central box was united to the other boxes with glass tubes, diagonally. The samples and the controls were distributed respectively in two opposed boxes containing 4 g of treated and untreated diet with solvent alone. The solvent used was hexane. In the central box forty adults were released. After 24 h the number of adults present at the boxes were counted. Each concentration was tested three times. Preference Index was calculated as PI= (% of insects at treated diet)-(% of insects at untreated diet) / (% of insects at treated diet)+(% of insects at untreated diet) where PI = -1 to – 0,10, repellent plant; PI= -0,10 to +0.10, Neutral plant and PI= +0,10 to +1, attractant plant. At 0.04% PI was -0.42 and at 0.4 PI was -0.77.