ADIPOSE TISSUE OF HYPOTHYROID RATS REDUCES PROLIFERATION AND MIGRATION OF MAMMARY TUMOR CELLS

ZYLA, LEILA; ÁVILA MANIERO MARÍA ÁNGELES; SASSO CORINA VERÓNICA; CARÓN RUBÉN; CALLEGARI EDUARDO; SANTIANO FLAVIA; PISTONE CREYDT VIRGINIA; LÓPEZ FONTANA CONSTANZA

Mendoza

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Universidad Nacional de Cuyo

In order to study the involvement of the adipose tissue as possible intermediary between thyroid states and breast cancer, we evaluate changes in the viability, adhesion and migration in ex vivo cultures of mammary tumors and in tumor and non-tumor mammary epithelial cell lines incubated with conditioned media (CMs) from mammary adipose tissue explants (TAM) of hypothyroid (HypoT) and euthyroid rats (EUT). Female Sprague‑Dawley rats were treated with a dose of DMBA dimethylbenzathracene (15 mg/rat) at 55 days of age and were then divided into two groups: HypoT (0.01% 6-N-propyl-2-thiouracil in drinking water, n=10) and EUT (untreated control, n=10). At sacrifice, TAM was obtained and the CMs were collected after 24 h of incubation with M199. Also, ex vivo cultures of mammary tumors from EUT were performed. Ex vivo cultures, MCF-10A, MCF-7 and MDA-MB-231 cells were grown and incubated with TAM-CMs of HypoT and EUT rats. Viability, adhesion and migration ability of cells were quantified. Soluble components present in CMs were identified by proteomics. Proteins were separated in polyacrylamide gels. An aliquot from CMs was lyophilized and the identification of proteins from complex samples was performed by two-dimensional nano-liquid chromatography (2D-nanoLC)-mass spectrometry (MS/MS). Finally, data was analyzed with ProteoIQ (Premier Biosoft) software. The CMs-TAM-HypoT contained fewer proteins that CMs-TAM-EUT (p