Estrogen Chronic administration effects on motor behavioral activity assessed by bioinformatic tools on different stages of a neurodegenerative process in adult male rats

M P BONACCORSO MARINELLI; RJ CABRERA; FE NIETO GRIMALT; MJ LEDESMA

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Congreso; LXI reunión anual de la sociedad de investigación clínica; 2016

We analyze estrogen actions in vivo in adult hemiparkinsonian male rats lesioned with 6-hydroxydopamine (6-OHDA) by bioinformatic assessing motor behavior on different stages of the neurodegenerative process. On postnatal day 60 (PND), 20 rats were injected with 6-OHDA in left corpus striatum (HP). From PND67 to 77, 10 HP rats received chronic treatment with 17b-Estradiol (E= 0.1 μg/kg/day s.c) and 10 got oil as vehi- cle. Other group (C) n=9 had the same surgery without 6-OHDA and oil treatment.Motor behavioral activity of all groups was video recorded and evaluated with automated softwares. Open Field Test (OFT) was con- ducted on PND74 without drug induction (NDI) and, on PND88 after amphetamine injection (AMPH). After 48h Forced Swimming Test (FST) NDI was evaluated.OFT total time moving after AMPH significantly increa- sed (p