IN VITRO AND IN VIVO STUDY OF Tessaria absinthioides (HOOK. & ARN.) DC CRUDE AQUEOUS EXTRACT CYTOTOXIC EFFECTS.

NARDIN AD; PERSIA FA; HAPON MV; HAPON MB; GAMARRA LUQUES C

San Luis

Congreso; XXXII Reunión de la Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

T. absinthioides (Asteracea) grows in many South America countries as Chile, Perú, Bolivia, Paraguay, Uruguay and Argentina. Is native flora of Mendoza, and have several reports of its use in folkloric medicine. This work aim was to analyze the cytotoxic properties of leaves aqueous extract against breast cancer cell line MCF-7 and Sprague Dawley strain rats. Crude extract was obtained autoclaving the leaves in distilled water; acute and chronic in vitro activity was studied by trypan blue exclusion assay and clonogenicity determination; in vivo toxicity was analyzed in healthy males and females by extract intragastric administration during 28 days at doses up to 1000 mg/kg/day. Acute extract treatment affect proliferation and viability of MCF-7 cells determining an IC50 value of 14.4 microgr/ml and a DL50 of 21.96 microgr/ml. Clonogenic survival was significantly reduced with doses 10 microgr/ml. There were no signs of toxicity that obligate to interrupt treatment before the 28 days. Growth curve in males shows a diminished slope but final weight reduction did not show a significant difference in respect to control group. Hematocrit in males and red cells count in both sexes were significantly increased. Serological levels of glucose, GOT (ASAT), urea and creatine did not show changes with treatment. In conclusion, cell line treatment outcomes were acute cytotoxic effects with persistent cell damage induction; while subchronic toxicity assays in animals, only determine scarce changes in biological parameters without evidence of severe deleterious effects. Altogether, the presented results make T. absinthioides aqueous extract a promise of natural product for cancer research.