PIOGLITAZONE PREVENTS OXIDATIVE DAMAGE AND INHIBITS VISFATIN EXPRESSION IN AORTIC PERIVASCULAR ADIPOSE TISSUE

ISABEL M. QUESADA; JIMENA CEJAS; MARÍA TERESA LÓPEZ; BEATRIZ CANNIZO; ANALÍA REDONDO; CLAUDIA CASTRO

San Luís

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo 2012; 2012

Perivascular adipose tissue (PVAT) has been shown to secrete a wide variety of adipokines,including visfatin and produces oxygen radicals via NADPH oxidase system. Thus, PVATprovides an important site of control of vascular (dys)function in obesity and type 2 diabetes(T2DM). Pioglitazone (PIO) is an insulin sensitizer which is currently approved for treatment ofT2DM. We analyzed the effect of PIO on the oxidative stress, the expression of p22 and Nox4,NADPH oxidase complex subunits, and visfatin gene expression in apolipoprotein E-deficientmice (ApoE-KO). Mice were fed with fructose overload (FF, 10% w / v) for 8 weeks and treatedwith PIO the latest 4 weeks We analyzed plasma biochemical variables and lipid peroxidationdetermined by thiobarbituric acid reactive species (TBARS). Visfatin, p22 and Nox4 geneexpression were determined in ApoE-KO PVAT. Glycaemia, insulinemia, triglyceridemia andTBARS increased significantly in the FF group and PIO prevented such increases. Visfatin andFF-induced p22 and Nox4 expression were significantly diminished by PIO in aortic PVAT. Ourresults show that in ApoE-KO mice, PIO improves insulin sensitivity, diminishes oxidativedamage provoked by FF treatment and induced suppression of visfatin, a pro-inflammatory adipokine.