EFECTO DEL HIPERTIROIDISMO EN LA CARCINOGÉNESIS PROSTÁTICA EN RATONES

GOMEZ, S; LOPEZ LAUR, JD; LOPEZ FONTANA, C; GINEVRO, PM; CANO, R; SANCHEZ, V; REY, LR; SANTIANO, F; MACKERN, JP; CARÓN, RW

Mendoza

Jornada; XXXVIII JORNADAS CIENTÍFICAS DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2021

Sociedad de Biología de Cuyo

Thyroid hormones are essential for the development and metabolism of normal and tumor prostate tissue. In vivo preclinical studies have found controversial effects of thyroid hormones on prostate cancer (PCa) development. The aim of this study was to determine the influence of hyperthyroidism on the incidence and progression of PCa. C57BL/6 male mice were subcutaneously inoculated with 5 x 106 TRAMP-C1 cells/200 µl in the left flank. Mice were randomly divided into 2 groups: hyperthyroid (Hyper, subcutaneous injection of 10 µg/mice/day thyroxine; n=10) and euthyroid as controls (EUT; n=10). Animals were observed daily until the appearance of palpable tumors. Latency and progression of tumors were determined. The mayor (DM) and minor (dm) diameters of tumors were measured using a caliper and tumor volume was calculated. At sacrifice, tumors were obtained for histological analysis (mitotic/apoptotic ratio) and immunohistochemistry (caspase 3 and PCNA). Statistical analysis was performed using Student T test or with U Mann Whitney test depending on the normality of the variable. All the animals developed PCa and the latency of onset of tumors was similar in the Hyper (39.33 + 2.09 days) and EUT group (37.71 + 5.6 days). However, Hyperthyroidism accelerated the tumor growth compared to controls (68.42 + 22.97 vs. 28.23 + 7.87 mm3/day, respectively; p