UHPLC–Q/Orbitrap/MS/MS fingerprinting and antitumoral effects of Prosopis strombulifera (LAM.) BENTH. queous extract on allograft colorectal and melanoma cancer models

RINALDINI, ESTEFANÍA; BÓRQUEZ, JORGE; GAMARRA-LUQUES, CARLOS; PERSIA, FABIO ANDRÉS; SIMIRGIOTIS, MARIO; MACKERN-OBERTI, JUAN PABLO; TRONCOSO, MARIANA ELIZABETH; TAPIA, ALEJANDRO; HAPON, MARÍA BELÉN

Heliyon

ELSEVIER GMBH

Año: 2020 vol. 6 p. 1 - 11

2405-8440

The aqueous extract of the Argentinean native plant, Prosopis strombulifera (PsAE), presents cytotoxicity againsthuman cancer cell lines by inducing cytostasis, necrosis and apoptosis; with diminution of clonogenic survival;without genotoxic effects nor oral animal toxicity. Until now, the chemical extract composition and its in vivoantitumoral properties remain unknown; these studies are the aim of the current work. The PsAE was characterizedby chemical fingerprinting and the metabolome was identified by tandem UHPLC-PDA-HESI-Q-orbitrap®mass spectrometry. Colorectal tumors were induced by DMH administration and melanomas resulted from B16-F0S.C. cells injection; then, animals were treated orally with PsEA. To correlate in vivo results with in vitro cytotoxicity,B16-F0 cell were cultured to determine: cell proliferation and viability by dye exclusion assays, MTT andCFSE dilution; cell cycle distribution by flow cytometry; and immunoblotting of p21cip1, PCNA, cleaved caspase 3,cleaved PARP and TUBA1A. Based on UHPLC-OT-MS and PDA analysis, twenty-six compounds were identified,including: 5 simple organic acids, 4 phenolic acids, 4 procyanidins, 11 flavonoids, and 2 oxylipins. On C57BL6mice, PsAE significantly increases the median survival on colorectal cancer and reduces the final volume andweight of melanomas. Over cultured cells, the treatment induce over-expression of p21, cytostasis by G2/M cellcycle arrest and apoptosis; while, on in vivo melanomas, treatment up-regulates p21 and slightly decreases PCNA.In conclusion, PsAE is composed by phenolic compounds which demonstrate cytotoxic and antitumoral propertieswhen is orally administrated. Presented results support future research of PsAE as a potential phytomedicine forcancer treatment.