COMPARATIVE METASTATIC AND NONMETASTATIC TUMOR RESPONSE TO HYALURONIC ACID TREATMENT. ROLE OF BETA-CATENIN AS A POSSIBLE MEDIATOR

SOFIA VALLA; GIANINA DEMARCHI; DAIANA VITALE; DAIANA DEL DAGO; CAROLINA CRISTINA; LAURA ALANIZ

Ciudad de Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

SAIC; SAIB; SAI; SAA; SAB; SAFE; SAFIS; SAH; SAP

Tumor microenvironment has a significant role in tumor malignancy, modulating several functions as angiogenesis. In this work we studied the relation of the extracellular matrix component, Hyaluronic acid(HA) and the protumoral signaling pathway Beta-Cateninand the effect of that interaction in angiogenic processes in tumors of different behavior. We used the metastatic breast cancer MDAMB-231 cells and the nonmetastatic prolactinoma MMQ cells. Cells were treated with HA (20 mg/ml) or the Wnt synthesis inhibitor, IWP-2 (5uM) for 48hs. Protein levels were analyzed by WB or FC and VEGF biosynthesis measured by ELISA. We found that the HA-receptor CD44 has low expression in MMQ cells (3%) while it was high in MDA cells (98%). HA induced Beta-Catenin expression in MDA cells (p