Preventive effect of a probiotic administration on the metabolic alterations caused by a fructose rich diet intake.

ZUBIRÍA G; GAMBARO S; CARASI P; RUMBO M; SERRADELL MA; GIOVAMBATTISTA A

Mar del Plata

Congreso; SAI-SAIC-SAFE 2016; 2016

SAI-SAIC-SAFE

There is large evidence that gut microbiota modulates metabolic disorders associated with obesity. We evaluated the preventive effect of a probiotic Lactobacillus administration on the metabolic alterations caused by a fructose rich diet (FRD). Four different Swiss mice groups were studied (n=10), CTR: mice were given tap water, FRD: mice were given 20% w/v fructose in the drinking water, and two similar groups that received by gavage 10^8 CFU of Lactobacillus kefiri CIDCA 8348 every 48 hs (CTR-Lk or FRD-Lk, respectively). Caloric intake and body weight were recorded every 48 hs. After 6 weeks, mice were euthanized and plasma samples were collected. Epididymal adipose tissue (EAT) was dissected, weighted and processed for qPCR and cellular CTR-Lk) and revert to control levels in FRD-Lk group (P