Characterization of the residual allergenicity of milk substitutes employed in the treatment of cow's milk allergy.

DOCENA. G.H.; ROZENFELD, P.A.; SERRADELL, M.A.; AÑÓN, M.C.; FOSSATI, C.A.

Bruselas, Bélgica

Congreso; XVIIIth Congress of the European Academy of Allergology and Clinical Immunology; 1999

European Academy of Allergology and Clinical Immunology

The treatment of the cow´s milk allergy (CMA) is avoidance of exposure to the sensitizing allergen. For this purpose, our aim was to analyze the presence of residual allergenic components in milk from goat, sheep soybean and in partially and extensively milk protein hydrolysates. By SDS-PAGE and immunoblotting (Ib), employing antisera and specific monoclonal antibodies, we could identified peptides, casein monomers and whey proteins in hypoallergenic substitutes. Besides, cross-reactives components between CM and soybean formula was also detected. The allergenicity of these components was assessed by Ib and EAST, analyzing the binding capacity to IgE from CMA patients. Moreover, a sequential ELISA was performed to quantify these residual components. Our results suggest that the extensively casien hydrolysates contain a low residual allergenicity in comparison with the other substitutes analyzed. Goat, sheep and soy formulas showed croos-reactive components with cow´s milk. Therefore, an appropiate diagnosis with the individual sensitization pattern, and the milk protein-specific residual allergenicity should be take into account for the prevention of CMA.