Lactobacillus kefiri modulates inflammation and restores microbiota dysbiosis in a mice DSS-induced colitis model

CARASI P; RACEDO S; JACQUOT C; SERRADELL M; URDACI M

Lisboa

Simposio; Microbiome and Host Health; 2013

Cell

Lactobacillus kefiri is an acid lactic bacteria species retrieved in the kefir, a fermented milk. The objective of this work was to evaluate the effect of L.kefiri CIDCA 8348 (Lk) administration in a colitis model induced by dextran sulfate sodium (DSS) in mice. Six weeks old Swiss mice were separated in four groups: control mice, Lk mice (received daily 108 cfu/mice of Lk), DSS mice (received DSS 3% w/v) and DSS-Lk mice (received Lk and DSS). Disease activity index was significantly lower in DSS-Lk mice than in DSS mice. Gene expression and histological studies were consistent with these findings. Regarding microbiota composition, DSS treatment modified Bacteroides, lactic acid bacteria, coliforms, Enterococcus, and Prevotella populations. Lk administration restored the microbiota populations towards these of the control mice. Only bacteroides group was not diminished with Lk administration. The observed dysbiosis restoration could explain the protective effect of Lk administration in this model.