GESTATIONAL ADMINISTRATION OF PROBIOTIC LENTILACTOBACILLUS KEFIRI ALTERS CYTOKINE RESPONSE AND STRUCTURAL PLACENTA ALTERATIONS IN A MOUSE MODEL OF PRETERM BIRTH

DIBO MJ; VALEFF NJ; POZO ALVAN MI; SERRADELL MA; VENTIMIGLIA MS; JENSEN CF

Mar del Plata

Congreso; Reunión Conjunta SAIC - SAI & FAIC - SAFIS 2022; 2022

SAIC - SAI & FAIC - SAFIS

Fetal well-being relays on an adequately function¬ing placenta. It has been demonstrated that placenta dysfunction is associated with severe pregnancy outcomes, increasing stillbirth rates as well as health risk for mother and neonate/child. We have previously reported that prophylactic treatment with probiotic Lentilactobacillus kefiri (Lk48) was highly effective in preventing lipopolysaccharide (LPS)-induced preterm birth and stillbirth in mice, favoring the good health and development of progeny. Moreover, we showed that Lk48 treatment ameliorated changes in placental labyrinths circulation by reducing the thickening of interhaemal membranes and neutrophil infiltration.The aim of the present study was to assess the effects L. kefiri on secretion profiles of cytokines from the placenta as well as vascular space quantification as a measure of the biological function in a mice model of LPS-perturbed pregnancies.C57BL/6 females were treated every 48h by oral gavage during a week with Lk48. Next, females were mated with BALB/c males. On gestational day (gd) 16, females were challenged with LPS. Tissue samples from placenta were harvest 18h after LPS injection. Levels of cytokines were evaluated on the supernatant from placental explants using ELISA kits. Histological examination and vascular space quantification of placental labyrinth were assessed by image analysis using ImageJ (www.imagej.nih.gov/ij/).Treatment with Lk48 altered cytokine production when compared to Control (t-test p