Change in lipid composition of Bradyrhizobium cell enveloped reveal a rapid response to water deficit involving lysophosphatidylethanolamine synthesis in outer membrane

CESARI ADRIANA; PAULUCCI N; BIASUTTI ALICIA; DARDANELLI M.S

Buenos Aires

Congreso; LIII Reunión Anual de la Sociedad Argentina de Investigación SAIB; 2017

SAIB

Cell enveloped is the primary targets affected by variations in themedium. Membrane behavior of Bradyrhizobium sp. SEMIA6144during adaptation to water deficit induced by polyethylene glycol(PEG) was evaluated. B. sp. SEMIA6144 was exposed to PEGshock (1 h, 5 h and 24 h).To investigate if PEG caused changes in cell morphology weused AFM. Fractions of internal and external cell membrane wereobtained and lipids were extracted. The fatty acids (FA) were analyzedby GC and phospholipid (PL) were identified by TLC. For itsquantification, [1-14C] sodium acetate was added to culture. Fluiditymembrane cell and multilamellar vesicles (MVLs) from lipids cellwas determined by measuring fluorescence polarization of DPH. Todetermine the PLA activity during PEG shock, outer membrane wasused as enzyme source.A dehydrating effect on the morphology of the cell surface as wellas a fluidizing effect on the membrane was observed 10 min afterPEG shock (p