Storage of Chinchilla lanigera semen at 4?aC for 24 or 72 hours with two different cryoprotectants.

CARRASCOSA RE; MARTINI AC; PONZIO MF; BUSSO JM; PONCE AA; LACUARA JL

criobiology

Elsevier Science Ltd.

Lugar: York, UK.; Año: 2001 vol. 42 p. 301 - 306

Resumen (copia del enviado a la editorial): The objectives of this study were to: (a) test the functional activity of Chinchilla lanigera spermatozoa suspendedin either glycerol or ethylene glycol, cooled to 4°C, and stored for 24 or 72 h and (b) investigate, after these cooling periods, the effects of incubating sperm at 37°C (for 4 h) upon sperm functional activity. The ejaculate was mixed with the cryoprotectant medium (at 1 M final concentration) and cooled to 4°C. After warming, sperm motility, sperm viability, hypoosmotic swelling test results, and acrosomal integrity were significantly higher for samples containing ethylene glycol than for those in glycerol, stored for 24 or 72 h, and then assayed after 0 or 4 h incubation at 37°C. A significant reduction of sperm motility and viability was detected only when the glycerol cryoprotectant agent was employed, compared to the fresh samples. These results clearly indicate that under our experimental conditions, ethylene glycol is a better protectant for sperm storage than glycerol.