EVALUATION OF THE ANTINOCICEPTIVE EFFECT OF MORPHINE IN A MODEL OF NEUROPATHIC PAIN IN MALE AND FEMALE MICE

PEREZ V; CANERO E.M.; BALERIO G. N

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021 SAIC - SAI - AAFE - NANOMED-ar. Segundo Congreso Virtual.; 2021

Morphine is one of the most widely used analgesics in the treatment of moderate and severe pain. However, its clinical use in long-term chronic pain treatment is limited by the enormous addictive potential. The co-administration of morphine with other drugs that enhance the analgesic effect and reduce its reinforcing properties, could be an alternative in pain treatment with opioids.In the present study, we propose to determine the lower effective dose of morphine to ameliorate the nociceptive threshold by using the partial sciatic nerve ligation (PSNL) in male and female Balb/C mice, a widely used model of neuropathic pain. The Von Frey test (VFT) was performed in order to evaluate mechanical allodynia by calculating the nociceptive threshold (g). First, mice were habituated to the environment of the experiment during 4 days. After the habituation period, baseline responses were measured and surgery of the right paw was performed in a group of animals with PSNL (PSNL group), and surgery without PSNL was executed in another group (Sham group). On day 9 after surgery, VFT was performed after administration of morphine (1, 3, 9 mg / kg, i.p.) or saline solution as vehicle.Finally, three-factor ANOVA (sex, surgery, treatment) was applied with Tukey´s post-hoc test, using a p