INVESTIGADORES
BUSTAMANTE Ana Victoria
artículos
Título:
Subtyping of STEC by MLVA in Argentina
Autor/es:
ANAV. BUSTAMANTE; ANDREAM. SANSO; ALBERTOE. PARMA; PAULAM.A. LUCCHESI
Revista:
Frontiers in Cellular and Infection Microbiology
Editorial:
Frontiers
Referencias:
Año: 2012 vol. 3 p. 1 - 4
ISSN:
2235-2988
Resumen:
Shigatoxin-producingEscherichiacoli(STEC)causesserioushumanillnesssuchashemolyticuremicsyndrome(HUS).Argentinahastheworld?shighestrateofthissyn-drome,whichistheleadingcauseofacuterenalfailureamongchildren.E.coliO157:H7isthemostcommoncauseofHUS,butasubstantialandgrowingproportionofthisillnessiscausedbyinfectionduetonon-O157strains.Multiple-locusvariable-numbertandemrepeatanalysis(MLVA)hasbecomeanestablishedtechniquetosubtypeSTEC.ThisreviewwilladdresstheuseofroutineSTECsubtypingbyMLVAinordertotypethisgroupofisolatesandtogetinsightintothegeneticdiversityofnativeSTEC.WithregardtotheseobjectiveswemodifiedandadaptedtwoMLVAprotocols,oneexclusiveforO157andtheother,agenericE.coliassay.Atotalof202STECisolates,fromdifferentsourcesandcorrespond-ingto20serotypes,havebeenMLVAgenotypedinourlaboratory.Inourexperience,MLVAconstitutesaverysensitivetoolandenablesustoperformanefficientSTECsubtyping.ThediversityfoundinmanyserotypesmaybeusefulforfutureepidemiologicalstudiesofSTECclonality,appliedtoO157aswellastonon-O157isolates.
