Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA)

BUSTAMANTE AV, LUCCHESI PMA, PARMA AE

BRAZILIAN JOURNAL OF MICROBIOLOGY

Sociedad Brasilera de Microbiología

Lugar: San Pablo; Año: 2009 vol. 40 p. 927 - 929

1517-8382

ABSTRACT The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.