Impact of exogen Ghrelin on early embryo development and implantation in mice.

LUQUE EM; VINCENTI LM; RUIZ RD; FIOL DE CUNEO M; MARTINI AC

San Juan

Congreso; Reunión Anual de la Sociedad de Biología Argentina; 2011

Embryos and endometrium express Ghrelin (Ghr) and its receptor. Our study evaluated the impact of Ghr and/or an antagonist (Ant=(D-Lys3)GHRP-6; 6nmol/animal/día) injection on embryo development and implantation. Adult female mice were injected (sc) from ovulation induction (Exp1) or from Day3 to Day7 of pregnancy (Exp2) with: Ghr1 (2nmol/animal/day), Ghr2 (4nmol/animal/day), Ghr2+Ant, Ant or isotonic solution (Con=control) and sacrificed at 80hs from estimated ovulation (embryos retrieved by uterine flushing) or at Day18 of pregnancy respectively. Exp1 (67-136 oocytes/group): Antagonist enhanced embryos recovery from uterus (65±17%; p