Maternal and postnatal high-fat diets with high omaga 3:omega 6 ratios affect the reproductive performance of male offspring in the mouse

BIANCONI S; STUTZ G; SOLÍS R; MARTINI AC; VINCENTI LM; PONZIO MF; LUQUE EM; AVENDAÑO C; QUIROGA P; SANTILLÁN ME

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2018 vol. 30 p. 1491 - 1502

1031-3613

High-fat diets (HFDs) are an acknowledged risk factor for male subfertility, but the underlying mechanismsremain unclear. In the present study we compared the effects of two HFDs with different v6 : v3 ratios, one enriched withsoy oil (SOD; v6 :v3 ¼ 9.62) and another enriched with sunflower oil (SFOD; v6 : v3 ¼ 51.55), with those of acommercial diet (CD; v6 : v3 ¼ 19.87), supplied from pregnancy to adulthood, on morphometric parameters andreproductive performance in adult male mice (recommended v6 :v3 for rodents ¼ 1?6). Bodyweight was significantlyhigher in the SFOD than CD group, and relative testicular weight was significantly lower in the SFOD than the other twogroups. SFOD altered sperm performance: it reduced sperm viability (mean s.e.m.; 76.00 1.35% vs 82.50 1.45%and 80.63 1.00% in the SFOD vs CD and SOD groups respectively; P , 0.05) and increased the percentage of immaturespermatozoa (71.88 7.17% vs 51.38 5.87% and 48.00 5.72% in the SFOD vs CD and SOD groups respectively;P , 0.05). The epididymal v6 : v3 ratio was higher in the SFOD versus CD and SOD groups, whereas the unsaturationindex was higher in the SOD and SFOD groups than in CD group. Sperm membrane integrity was diminished in both theSOD and SFOD groups, but there was no difference in sperm reactive oxygen species production in these two groupscompared with the CD group. The fertilisation rate was lower in the SFOD compared with the CD and SOD groups. Inconclusion, although both HFDs affected sperm quality, the fertilising ability was more altered by the excessive dietaryv6 :v3 ratio than by the net v6 content.