Inhibitory effects of ghrelin on sexual behavior: role of the peptide in the receptivity reduction induced by food restriction in mice.

BERTOLDI ML; LUQUE EM; CARLINI VP; VINCENTI LM; STUTZ G; SANTILLÁN ME; RUIZ RD; FIOL DE CUNEO M; MARTINI AC

HORMONE AND METABOLIC RESEARCH

GEORG THIEME VERLAG KG

Año: 2011 vol. 43 p. 494 - 499

0018-5043

Ghrelin (Ghr) is a gut/hypothalamus peptide with inhibitory actions on reproductive physiology; however, there are no previous reports of its role on estrous behavior. Under the hypothesis that the increase of plasma Ghr during food restriction (FR) is responsible for receptivity reduction, we intended to evaluate the receptivity percentage of female mice subjected to: exp1) acute and chronic FR and Ghr administration (3 nmol/animal/day, s.c.) and exp2) the co-administration of a ghrelin antagonist (ant=(D-Lys3)GHRP-6; 6 nmol/animal/day s.c.). All females were ovariectomized, primed with steroids, trained and randomly subjected every week to each one of several protocols, followed by a behavioral test. Experiment 1 (n=8): basal, no treatment; acute FR (aFR), 24 h fasting; chronic FR (cFR), 50% FR for 5 days; acute ghrelin (aGhr), Ghr 30 min before test and chronic ghrelin (cGhr), Ghr for 5 days. Except for cGhr, all treatments significantly decreased the % of receptivity (Mean±SEM): basal 61.9±6.0, aFR 33.1±8.1, cFR 18.8±7.7, aGhr 45.6±10.6, p< 0.05 vs basal. In exp2 (n=11), except for cFR+ant (55.0±6.4) the co-administration of the antagonist reversed the deleterious effects detected in exp1: basal 70.9±5.4; aFR+ant 72.3±7.6; aGhr+ant 73.6±4.7. As expected, the administration of vehicle or antagonist alone did not modify receptivity. Besides, we found a significant correlation between % of body weight loss and % of receptivity reduction (r= 0.62, p= 0.0004). This is the first study demonstrating that ghrelin is able to inhibit female mice sexual behavior and that is involved, at least in part, in receptivity reduction after food scarcity.