Signal molecules in the peanut?bradyrhizobia interaction

TANIA, TAURIAN; MORÓN, BELÉN; SORIA-DÍAZ, MARÍA; ANGELINI, JORGE; TEJERO-MATEO, PILAR; GILL-SERRANO, ANTONIO; MEGÍAS, MANUEL; FABRA, ADRIANA

ARCHIVES OF MICROBIOLOGY

SPRINGER

Lugar: Berlin; Año: 2008 p. 345 - 356

0302-8933

Main nodulation signal molecules in the peanut bradyrhizobia interaction were examined. Flavonoids exuded by Arachis hypogaea L. cultivar Tegua were genistein, daidzein and chrysin, the latest being released in lower quantities. Thin layer chromatography analysis from genistein induced bacterial cultures of three peanut bradyrhizobia resulted in an identical Nod factor pattern, suggesting low variability in genes involved in the synthesis of these molecules. Structural study of Nod factor by mass spectrometry and NMR analysis revealed that it shares a variety of substituents with the broad host range Rhizobium sp. NGR234 and Bradyrhizobium spp. Nodulation assays in legumes nodulated by these rhizobia demonstrated diferences between them and the three peanut bradyrhizobia. The three isolates were classiWed as Bradyrhizobium sp