INVESTIGADORES
ANGELINI Jorge Guillermo
artículos
Título:
Sequence and expression analysis of putative Arachis hypogaea (peanut) Nod factor perception proteins
Autor/es:
IBAÑEZ, F.; ANGELINI, J.; FIGUEREDO, MS.; MUÑOZ, V.; TONELLI, M.; FABRA, A.
Revista:
JOURNAL OF PLANT RESEARCH
Editorial:
SPRINGER TOKYO
Referencias:
Lugar: Tokyo; Año: 2015
ISSN:
0918-9440
Resumen:
Peanut, like most legumes, develops a symbi-
otic relationship with rhizobia to overcome nitrogen limita-
tion. Rhizobial infection of peanut roots occurs through a
primitive and poorly characterized intercellular mechanism.
Knowledge of the molecular determinants of this symbiotic
interaction is scarce, and little is known about the mol-
ecules implicated in the recognition of the symbionts. Here,
we identify the LysM extracellular domain sequences of
two putative peanut Nod factor receptors, named AhNFR1
and AhNFP. Phylogenetic analyses indicated that they cor-
respond to LjNFR1 and LjNFR5 homologs, respectively.
Transcriptional analysis revealed that, unlike LjNFR5,
AhNFP expression was not induced at 8 h post bradyrhizo-
bial inoculation. Further examination of AhNFP showed
that the predicted protein sequence is identical to GmNFR5
in two positions that are crucial for Nod factor perception
in other legumes. Analysis of the AhNFP LysM2 tridimen-
sional model revealed that these two amino acids are very
close, delimiting a zone of the molecule essential for Nod
factor recognition. These data, together with the analysis
of the molecular structure of Nod factors of native peanut symbionts previously reported, suggest that peanut and
soybean could share some of the determinants involved in
the signalling cascade that allows symbiosis establishment.