First characterization of Leishmania species from Argentina based on the heat shot protein 70 gene (HSP70)

LAUTHIER, JUAN JOSÉ; RUYBAL, PAULA; CAJAL, PAMELA; BARROSO, PAOLA ANDREA; HOYOS, CARLOS LORENZO; HASHIGUCHI, YOSHIHISA; CIMINO, RUBÉN; MARCO, JORGE DIEGO; KORENAGA, MASATAKA

Otro; 87th Annual Meeting of the Japanese Society of Parasitology; 2018

The Japanese Society of Parasitology

American tegumentary leishmaniasis (ATL) is a group of zoonotic diseases caused by kinetoplastid flagellates of the genus Leishmania. ATL is endemic in northern Argentina, and both the largest number of reported cases (53,1%) and the highest incidence have been found in the north of Salta Province, which represents only 0.7% of the country's population, being the primary locus of CL and MCL. In Argentina, the characterization using cytB sequence is a validated method for the indentification of the Leishmania spp. In accordance with the diversity suggested by MLEE, cytB gene sequences have assigned two different genotypes for L. (V.) braziliensis named AB-a and Ab-2. HSP70 gene has been widely used for Leishmania spp. characterization and became one of the mosto analyzed targets. In Argentina, there is no published work tha use this molecular marker for diversity description in local isolates from Argentina. Here, a total of 20 isolates from patients rom northwest Argentina and previously characterized with cytB were included in this study. All the isolates correspond to genotypes AB-1 and Ab-2 of L. (V.) braziliensis. The main objective of this work was to correlate the results obtained by the characterization of HSP70 with those of cytB, and to see if the genotypes Ab-1 and Ab-2 remain in both targets.