HEK293 CELL LINE BEHAVIOR IN CONTACT WITH HYDROGELS BASEDON PNIPAM

CAPELLA, VIRGINIA; RIVERO, R.; LIAUDAT, CECILIA; IBARRA, LUIS; BARBERO, CESAR; BOSCH, PABLO; RIVAROLA, CLAUDIA; RODRIGUEZ, N

Congreso; XXI Annual Scientific Meeting; 2018

Sociedad de Biología de Córdoba

Tissueengineeringisbasedonthecombineduseofcells,biomaterialsandsoluble factors inorder torepairdamaged tissues.In thelastdecades,thedesignofmaterialsthatserveasscaffoldforthegrowthanddevelopmentofcells,obtainedfromdifferenttissuesandorgans,hastakenrelevance.Biocompatiblehydrogelsareakindofmaterialthathasgreatpotentialinbiomedicinebecauseitsstructureandpropertiesaresimilartotheextracellularmatrix.Inthepresentwork,thecellviabilityofhumanembryonickidney(HEK293)celllineexposedtodifferenthydrogels,basedonpoly-Nisopropylacrylamide(PNIPAM),wasevaluated.HEK293cellshavebeenwidelyusedincellbiologyresearchformanyyearsbecauseoftheirreliablegrowthandpropensityfortransfection.Celladhesiononhydrogelsurfaceswasalsoanalyzed.Thecytotoxicityofthecellsexposedfor48and96hwasevaluatedthroughtheMTTandneutralredtests.Thecellswereseededontohydrogelsandtheirgrowthonthesurfacewasobservedduring6daysthroughstainingwithHoechstandtoluidineblue.Theresultsindicatedthatallevaluatedhydrogelsdidnotproducetoxicityonthecellline.CellgrowthonPNIPAMsurfaceswasobserved.Theseresultssuggestthatthestudiedhydrogelsaregoodcandidates touseas scaffoldsforHEK293 cell growth forapplications inthe field