Bovine fetal Fibroblast growth inside 3D scaffold based on macroporous PNIPAM hydrogels

RIVERO, R.; CAPELLA, VIRGINIA; BOSCH P.; RODRIGUEZ, N; BARBERO, CESAR; RIVAROLA, CLAUDIA

Congreso; XXI Annual Scientific Meeting; 2018

Sociedad de Biología de Córdoba

Biomaterialsarebeingusedincellculturetechniquesasscaffoldstocreatetissuelikestructuresthatsimulatethemechanicalandphysiologicalfeaturesofinvivotissues.Biomaterialsbasedonhydrogelshavegreatpotentialfortissuereconstructionduetotheycouldemulateextracellularmembrane(ECM)providingsitesforadhesion,proliferationandevenmorecelldifferentiation.Inthiswork,weproposetostudybovinefetalfibroblastcellgrowthinside3DscaffoldsbasedonmacroporousPNIPAMhydrogels.Thehydrogelswerepolymerizedbycryogelation(at-18°C).Theporousmorphologywasobservedbyscanningelectronmicroscopy(SEM).Histologicalassayswerecarriedoutbysimplehematoxylinandeosinstaining. Bovine fetal fibroblasts(BFF)wereseededontohydrogelcylindersandthenthecellswereculturedfor30days.CellviabilitywasqualitativelystudiedusingtrypanblueassayandcellmorphologywasobservedbySEM.TheobtainedresultsindicatethatBFFscangrowinsidemacroporousPNIPAMhydrogelsmainlyadsorbedonmacropores-fluidbiologicinterphase. BFF adopt spheroid morphology in contrast to slape morphology adopted onPNIPAM surface.Highcellviabilityinsidehydrogelindicatesthatthesematerialscouldbeusedasscaffoldsforcellculturewithpotentialapplications intissueengineeringfield.