Efficient Transduction of Bovine Fetal Fibroblasts by an Adenoviral Vector

SCILINGO, ANA MARÍA; ORTEGA, NICOLÁS MATÍAS; BARRIONUEVO, BLANCA ELIZABETH; BRINGAS, MARIANA; BENITEZ, SABRINA; GUIDO, CAROLINA; OLMOS, MARÍA FLORENCIA; FILI, ALEJANDRO; FORCATO, DIEGO; BOSCH, P

Buenos Aires

Jornada; XII Jornadas Anuales de la Sociedad Argentina de Biología; 2010

Sociedad Argentina de Biología

Forced expression of signaling molecules and transcription factors has proven useful to manipulate cultured cells, e.g., to generate induced pluripotent stem cells. The study was aimed at examining the effect of FuGene (Roche), Lipofectamine 2000 (Invitrogen) and electroporation on transduction efficiency of bovine fetal fibroblasts (BFFs) by an adenoviral vector carrying the GFP gene (Ad-GFP; provided by C. Hereñú and R. Goya from INIBIOLP). BFF cultures were trypsinized 72 hs after transduction and the percentage of GFP positive cells was determined by cell counting. Addition of FuGene at a final concentration of 1.3% increased the percentage of GFP-expressing cells (control= 13.9% vs FuGene at 1.3%= 24.5%; p